Immunofluorescence

Make a colorful splash and upgrade your research with our high-quality, stringently validated antibodies for immunofluorescence (IF).

Directly conjugated CoraLite antibodies

Streamline your IF workflow and save precious hours in the lab with our directly conjugated CoraLite Primary antibodies. The perfect companion for multiplexing and IF experiments.

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Primary antibodies for IF

Over 13,000 polyclonal,monoclonal and recombinant primary antibodies against the protein of interest.

  • Polyclonal: Raised in rabbit
  • Monoclonal: Raised in mouse
  • Recombinant: Raised in rabbit
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Secondary antibodies

When choosing a secondary antibody for IF, be mindful of:

  • Host of your primary antibody E.g. rabbit; and
  • Your excitation/emission spectra when building a multiplex panel
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Nano-Secondary® reagents

Nano-Secondary® reagents use a novel class of secondary antibodies. They enable cleaner images with higher resolution and can be named the next level of secondary antibodies. Nano-Secondary® reagents consist of Nanobodies conjugated to Alexa Fluor® dyes, that bind to primary antibodies with high affinity in a species and subtype specific manner.

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Technical support for IF

High background? No staining? Autofluorescence? Find out how to optimise your IF protocol and solutions to many IF problems here.

Overview of IF protocol

overview of Immunofluorescence protocol

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