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Immunoprecipitation of Myc-tagged proteins – How …

ChromoTek Myc-Trap® is an effectiveness optimized tool for immunoprecipitation (IP/Co-IP) and affinity purification of Myc-tagged proteins. Depending on whether you use a single Myc-tag, i.e. 1xMyc (EQKLISEEDL) or a double Myc-tag fused to the protein of interest, i.e. 2xMyc (EQKLISEEDLEQKLISEEDL) the performance is slightly different. … – Read more

Analyze protein interactions live in mammalian …

Do you have GFP or RFP protein constructs and/or libraries? Do you want to screen agonists or antagonists? Do you want to save time and start your experiment without method set-up? Just do it. All you need is the ChromoTek F2H Kit Basic: Transfect the bait and the prey plasmids with the Platform Reagent into the F2H cells. … – Read more

How to prepare protein interaction partners for MS …

Immunoprecipitation (IP) followed by mass spectrometry (MS) analysis is a powerful method to identify interaction partners of a protein of interest. However, sometimes it can be difficult to obtain reliable results. Now, the combined use of ChromoTek GFP-Trap for immunoprecipitation and PreOmics iST sample … – Read more

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