F2H Cellular assays

Cellular protein-protein interaction assays

ChromoTek's Fluorescent Two-Hybrid (F2H®) Technology enables you to study protein-protein interactions (PPIs) in mammalian cells in a fast and simple way by conventional fluorescence microscopy. The direct optical readout is optimal for high-content analysis (HCA) and screening for PPI inhibitors.

Specificity
GFP-fusion proteins & RFP or mCherry-fusion proteins

Applications
Visualize and validate PPIs in an intracellular environment
Support biochemical PPI analysis by fluorescence imaging
Identify interacting protein domains
Monitor PPI dynamics in live cells upon treatments, determine inhibition kinetics
Screen for compounds inhibiting or activating PPIs

You just need an epi-fluorescence microscope. Analyze your GFP- and RFP-fusion proteins/ library or prevalidated assays for Androgen Receptor, p53/Mdm2 or p53/Mdm4.

F2H Kit Androgen Receptor

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CodeDescriptionSizePriceQty
Codef2hk-ar-wtDescriptionF2H®-Kit AR-N/C wt (Androgen Receptor, wt) contains: F2H®-BHK Cell line, 5x106 cells, Platform-ARLBD(wt)-ARNTD, 100µg, Positive Control, 30µgSizeF2H®-BHK Cell line: 5x106 cells, Platform-ARLBD(wt)-ARNTD: 100 µg, Positive Control: 30 µgPrice $ 1385
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Codef2h-ar-wtDescriptionPlatform-ARLBD(wt)-ARNTD (100 µg)Size100 µgPrice $ 525
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Codef2h-ar-w741lDescriptionPlatform-ARLBD(W741L)-ARNTD (100 µg)Size100 µgPrice $ 525
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Codef2h-ar-f876lDescriptionPlatform-ARLBD(F876L)-ARNTD (100 µg)Size100 µgPrice $ 525
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Codef2h-ar-t877aDescriptionPlatform-ARLBD(T877A)-ARNTD (100 µg)Size100 µgPrice $ 525
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Codef2h-ar-f876l/t877aDescriptionPlatform-ARLBD(F876L/T877A)-ARNTD (100 µg)Size100 µgPrice $ 525
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Codef2h-caDescriptionPositive Control (30 µg)Size30 µgPrice $ 120
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F2H Kit Basic

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CodeDescriptionSizePriceQty
Codef2hkDescriptionF2H®-BHK cell line (5x106 cells), 100 µg platform reagent, 30 µg control DNASizeF2H®-BHK Cell line: 5x106 cells, Platform reagent: 100 µg, Control DNA: 30 µgPrice $ 1230
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Codef2h-p100DescriptionPlatform reagent (100µg)Size100µgPrice $ 345
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Codef2h-c30DescriptionControl DNA (30 µg)Size30 µgPrice $ 120
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F2H Kits p53-Mdm2/4

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CodeDescriptionSizePriceQty
Codef2hk-p53/mdm2DescriptionF2H®-Kit p53/Mdm2 contains: F2H®-BHK Cell line (5x106 cells), Platform-p53-Mdm2, Control-p53SizeF2H®-BHK Cell line: 5x106 cells, Platform-p53-Mdm2: 100 µg, Control-p53: 30 µgPrice $ 1385
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Codef2hk-p53/mdm4DescriptionF2H®-Kit p53/Mdm4 contains: F2H®-BHK Cell line (5x106 cells), Platform-p53-Mdm4, Control-p53SizeF2H®-BHK Cell line: 5x106 cells, Platform-p53-Mdm4: 100 µg, Control-p53: 30 µgPrice $ 1385
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Codef2h-ppm2DescriptionPlatform-p53-Mdm2 (100 µg)Size100 µgPrice $ 525
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Codef2h-ppm4DescriptionPlatform-p53-Mdm4 (100 µg)Size100 µgPrice $ 525
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Codef2h-cpDescriptionControl-p53 (30 µg)Size30 µgPrice $ 120
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Two-hybrid screening

Two hybrid screenings are used for identification, confirmation and characterization of protein-protein or protein-DNA interactions in living cells. Since the development of the classic Yeast two-hybrid (Y2H) screen 30 years ago, many different two-hybrid systems have been developed.

PRODUCTS

High content analysis

High-content screening (HCS) comprises multiple cellular assays that are conducted in an automated, parallel manner to analyze a set of different biological parameters in live cells in a high throughput format. It is used in cell biology and drug discovery to find compounds that impact cellular processes, protein-protein interactions, and/or the phenotype of cells. Tested compounds include small molecules, peptides, and RNAi. Assays are usually based on fluorescence imaging. Next to automated microscopes for data acquisition also automated image analysis software is used, because huge data amounts are generated. HCS is also called high-content analysis (HCA) or cellomics.

PRODUCTS

Hear from our customers

“The F2H® approach is the missing link between biochemistry and cell biology”

Prof. Sir David Lane, p53 Lab, A*Star, Singapore (find more references here >)

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Only for research applications, not for diagnostic or therapeutic use!