GFP VHH, recombinant binding protein

Description
Alpaca anti-GFP VHH, purified unconjugated protein

Synonyms
GFP-Binding-Protein, GFP-Nanobody, and GFP-single domain antibody (sdAb)

Specificity
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and GFP-derivatives such as EGFP, CFP, YFP, BFP, and many more. For a full list, see
Fluorescent protein specificity table

Applications
ELISA
Immunofluorescence
Immunoprecipitation

ProductSizeCodePriceBuy
ProductGFP VHH, recombinant binding proteinSize250 µLCodegt-250Price $ 275
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Affinity
Dissociation constant KD of 1 pM

Specificity

GFP, EGFP, CFP, YFP, BFP and many more derivatives of GFP from Aequorea victoria

For Immunoprecipitation see: Fluorescent protein specificity table

For Immunofluorescence: eCFP, CFP, mCerulean, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine

Conjugation:
Unconjugated
Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction

Biophysical properties
Molecular weight MW: 13.9 kDa
Molecular extinction coefficient ε: 27,055 L·Mol−1·cm−1
Isoelectrical point pI: 9.4 (theoretical)

Form
Purified recombinant antibody fragment, monoclonal

Protein concentration
1 mg/mL

Storage buffer
25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide

Storage instructions
Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year.

Do not freeze.

Are the Nanobodies monoclonal or polyclonal?

The Nanobodies are recombinant/monoclonal.

What are the biophysical parameters of the Nanobodies?

NameMW (kDa)Molar Extinction Coefficient
(M-1 cm-1)
Theoretical pI
GFP VHH13.927.0559.4
GST VHH14.828.5458.2
Halo VHH14.823.0459.4
Histon VHH14.425.56510.2
MBP VHH14.127.05510.0
Mdm4/Hdmx VHH13.614.56510.1
MK2 VHH14.432.5559.7

mNeonGreen VHH

14.321.6808.0
Myc VHH13.523.0459.6
p53 C-term VHH14.834.1709.2
p53 N-term VHH13.020.0659.7
PARP1 VHH14.417.6709.2
RFP VHH14.930.0359.3
SNAP/CLIP-tag VHH13.124.0759.8
Spot VHH (bivalent)30.346.3405.3
TurboGFP VHH13.618.5759.5
Vimentin VHH26.748.15010.0

Do the Nanobodies have any tags?

Yes, the Nanobodies have a C-terminal His6-tag.

Does the GFP VHH bind to protein A or protein G?

The GFP VHH binds to protein A, but not to protein G.

Extraordinary stable & reliable binding

  • GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate
  • Fulfills highest requirements for antibody validation
  • Structure and function are characterized

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Only for research applications, not for diagnostic or therapeutic use!