GST-Trap

Description
The ChromoTek GST-Trap is a ready to use resin for immunoprecipitation/pulldown of GST fusion proteins.
The GST-Trap consists of a GST Nanobody /VHH coupled to agarose beads.

Specificity
Glutathione S-Transferase (GST) from Schistosoma japonicum

Applications
Immunoprecipitation (IP) / Co-IP
Mass spectrometry (MS)
On-bead enzyme assays
ChIP / RIP analysis

Formats/matrices
GST-Trap Agarose
GST VHH, recombinant binding protein

Synonyms

GST VHH, anti-GST VHH, GST-Binding-Protein, GST Nanobody, or GST single domain antibody (sdAb)

Benefit from GST-Trap for Immunoprecipitation (IP)

  • Effectively immunoprecipitates low expressed GST-fusion proteins
  • Considerably higher affinity than glutathione cellulose and other affinity resins
  • Strong binding even under harsh washing conditions
  • No heavy & light chains in your downstream application

Format/matrices & properties

Extraordinary stable & reliable binding

  • Dissociation constant KD of 1 nM
  • Compatible to harsh wash conditions:  4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
  • Pulldown of GST-fusion proteins at low concentrations
  • Fully applications validated and characterized

About GST

Glutathione S-Transferase (GST) from Schistosoma japonicum is a 220 aa (about 26 kDa) protein, which is commonly used as a protein tag for immunoprecipitation and affinity purification. GST may increase the solubility and expression level of the fusion protein (so-called solubilization tag). Furthermore, GST-tagged fusion proteins are used in protein-protein interaction assays. Applications require highly specific tools to isolate GST-fusion proteins.

Find the GST-Trap:

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Only for research applications, not for diagnostic or therapeutic use!