SNAP/CLIP-tag-Trap

Description

The ChromoTek SNAP/CLIP-tag®-Trap is an affinity resin for immunoprecipitation of SNAP-tag and CLIP-tag fusion proteins.

It comprises an anti-SNAP-tag and CLIP-tag Nanobody/ VHH conjugated to beads.

Specificity
SNAP-tag and CLIP-tag

Applications
Immunoprecipitation (IP) / Co-IP
Mass spectrometry (MS)
On-bead enzyme assays
ChIP / RIP analysis

Formats/matrices
SNAP/CLIP-tag®-Trap Agarose
SNAP/CLIP-tag® VHH, recombinant binding protein

Synonyms
anti-SNAP/CLIP-tag VHH, SNAP/CLIP-tag binding protein, SNAP/CLIP-tag Nanobody, or anti- SNAP/CLIP-tag single domain antibody fragment (sdAb)

Benefit from SNAP/CLIP-tag®-Trap for Immunoprecipitation (IP)

  • SNAP/CLIP-tag-Trap immunoprecipitates SNAP-tag- and Clip-tag- fusion proteins, no matter if covalently bound to ligands or not
  • Bound SNAP-tag- and Clip-tag- fusion proteins can be eluted without protease: No need for a protease cleavage site
  • SNAP/CLIP-tag-Trap can be used for affinity purification
  • Recombinant, validated VHH

Format/matrices & properties

The SNAP/CLIP-tag® VHH is either offered conjugated to agarose beads or uncoupled as purified protein.

Extraordinary stable & reliable binding

  • Dissociation constant KD of 1 nM
  • Compatible to the following wash conditions: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
  • Applications validated and characterized

Specificity

SNAP/CLIP-tag®-Trap recognizes SNAP-tag & CLIP-tag fusion proteins that are already bound to already bound to benzylguanine and  benzylcytosine derivatives.

About SNAP-tag and CLIP-tag

SNAP- and the CLIP-tag are mutants of the human DNA repair protein O6-alkylguanine-DNA alkyltransferase (hAGT)  and are designed to form covalent bonds with with benzylguanine and benzylcytosine derivatives.

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Only for research applications, not for diagnostic or therapeutic use!