Antibodies: Nanobodies/ VHHs

ChromoTek’s unconjugated Nanobodies or VHHs are well characterized and validated for the listed applications. Because the molecular weight of these single-domain antibodies (sdAb) is just about 15 kDa, VHHs are only one tenth the size of a conventional IgG antibody (150 kDa) or less than a third the size of a Fab fragment (50 kDa). In combination with their high chemical and thermal stability plus high affinity, these binding proteins are ideal for multiple types of affinity assays in biochemistry and microscopy, including immunoprecipitation, immunofluorescence, super resolution microscopy, surface plasmon resonance (SPR), biolayer interferometry (BLI), switchSENSE technology, etc.

GFP VHH, biotinylated recombinant binding protein

x
CodeDescriptionSizePriceQty
Codegtb-250DescriptionGFP-Binding-Protein, 1 mg / mLSize250 µLPrice $ 500
Qty+

GFP VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codegt-250DescriptionGFP-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

GST VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codest-250DescriptionGST-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

Halo VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codeot-250DescriptionHalo-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

Histone VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codett-250DescriptionHistone Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

MBP VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codembt-250DescriptionMBP-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

Mdm4/HdmX VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codeht-250DescriptionMdm4/HdmX-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

MK2 VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codemt-250DescriptionMK2-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

mNeonGreen VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codent-250DescriptionmNeonGreen-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

Myc VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codeyt-250DescriptionMyc-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

p53 C-term VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codept2-250Descriptionp53 C-term-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

p53 N-term VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codept-250Descriptionp53 N-term-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

PARP1 VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codext-250DescriptionPARP1-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

RFP VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codert-250DescriptionRFP-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

SNAP/CLIP-tag® VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codewt-250DescriptionSNAP/CLIP-tag®-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

Spot VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codeetb-250DescriptionSpot-Binding-Protein, 1 mg / mLSize250 µLPrice $ 455
Qty+

TurboGFP VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codetbt-250DescriptionTurboGFP-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
Qty+

Vimentin VHH, recombinant binding protein

x
CodeDescriptionSizePriceQty
Codevt-250DescriptionVimentin Binding-Protein, 1 mg / mLSize250 µLPrice $ 500
Qty+

Ligand capture and Affinity Determination Methods

Antibodies or nanobodies can be used to immobilize proteins on various surfaces, such as microtiter plates, beads or biosensors. Protein-protein interactions and affinity (kon/koff and KD values) can be measured with ligand capture-based assay setups, e.g. by surface plasmon resonance (SPR, BiacoreTM/GE Healthcare), biolayer interferometry (BLI, FortéBio/Pall) or the switchSENSE® technology (Dynamic Biosensors). As a prerequisite for these assays, the affinity of the antibody or Nanobody to the capture ligand has to be high enough to assure ligand binding during the time of the measurement.

ChromoTek’s anti-GFP Nanobody has an extremely high affinity to GFP, with a KD value of approx. 1 pM, as determined by switchSENSE. Note that the other Nanobodies from ChromoTek like the GST, MBP VHHs do have single digit nanomolar KD values, which also allow for high affinity binding in before mentioned applications. Therefore, it is a perfect tool for capturing GFP-tagged proteins for protein-protein interaction studies and affinity determination. Furthermore, its high stability allows regeneration and reuse of the biosensor for multiple measurements.

PRODUCTS

Download Whitepaper
Order Free Test-Sample
Email newsletter

Only for research applications, not for diagnostic or therapeutic use!