Antibodies: Nanobodies/ VHHs

ChromoTek’s unconjugated Nanobodies or VHHs are well characterized and validated for the listed applications. Because the molecular weight of these single-domain antibodies (sdAb) is just about 15 kDa, VHHs are only one tenth the size of a conventional IgG antibody (150 kDa) or less than a third the size of a Fab fragment (50 kDa). In combination with their high chemical and thermal stability plus high affinity, these binding proteins are ideal for multiple types of affinity assays in biochemistry and microscopy, including immunoprecipitation, immunofluorescence, super resolution microscopy, surface plasmon resonance (SPR), biolayer interferometry (BLI), switchSENSE technology, etc.

GFP VHH, biotinylated recombinant binding protein

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Code Description Size Price Qty
Code gtb-250 Description GFP-Binding-Protein, 1 mg / mL Size 250 µL Price $ 500
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GFP VHH, recombinant binding protein

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Code Description Size Price Qty
Code gt-250 Description GFP-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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GST VHH, recombinant binding protein

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Code Description Size Price Qty
Code st-250 Description GST-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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Halo VHH, recombinant binding protein

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Code Description Size Price Qty
Code ot-250 Description Halo-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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Histone VHH, recombinant binding protein

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Code Description Size Price Qty
Code tt-250 Description Histone Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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MBP VHH, recombinant binding protein

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Code Description Size Price Qty
Code mbt-250 Description MBP-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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MK2 VHH, recombinant binding protein

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Code Description Size Price Qty
Code mt-250 Description MK2-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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mNeonGreen VHH, recombinant binding protein

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Code Description Size Price Qty
Code nt-250 Description mNeonGreen-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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Myc VHH, recombinant binding protein

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Code Description Size Price Qty
Code yt-250 Description Myc-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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p53 C-term VHH, recombinant binding protein

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Code Description Size Price Qty
Code pt2-250 Description p53 C-term-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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p53 N-term VHH, recombinant binding protein

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Code Description Size Price Qty
Code pt-250 Description p53 N-term-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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PARP1 VHH, recombinant binding protein

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Code Description Size Price Qty
Code xt-250 Description PARP1-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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RFP VHH, recombinant binding protein

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Code Description Size Price Qty
Code rt-250 Description RFP-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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SNAP/CLIP-tag® VHH, recombinant binding protein

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Code Description Size Price Qty
Code wt-250 Description SNAP/CLIP-tag®-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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Spot VHH, recombinant binding protein

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Code Description Size Price Qty
Code etb-250 Description Spot-Binding-Protein, 1 mg / mL Size 250 µL Price $ 455
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TurboGFP VHH, recombinant binding protein

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Code Description Size Price Qty
Code tbt-250 Description TurboGFP-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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Vimentin VHH, recombinant binding protein

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Code Description Size Price Qty
Code vt-250 Description Vimentin Binding-Protein, 1 mg / mL Size 250 µL Price $ 500
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Ligand capture and Affinity Determination Methods

Antibodies or nanobodies can be used to immobilize proteins on various surfaces, such as microtiter plates, beads or biosensors. Protein-protein interactions and affinity (kon/koff and KD values) can be measured with ligand capture-based assay setups, e.g. by surface plasmon resonance (SPR, BiacoreTM/GE Healthcare), biolayer interferometry (BLI, FortéBio/Pall) or the switchSENSE® technology (Dynamic Biosensors). As a prerequisite for these assays, the affinity of the antibody or Nanobody to the capture ligand has to be high enough to assure ligand binding during the time of the measurement.

ChromoTek’s anti-GFP Nanobody has an extremely high affinity to GFP, with a KD value of approx. 1 pM, as determined by switchSENSE. Note that the other Nanobodies from ChromoTek like the GST, MBP VHHs do have single digit nanomolar KD values, which also allow for high affinity binding in before mentioned applications. Therefore, it is a perfect tool for capturing GFP-tagged proteins for protein-protein interaction studies and affinity determination. Furthermore, its high stability allows regeneration and reuse of the biosensor for multiple measurements.

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Only for research applications, not for diagnostic or therapeutic use!