GFP VHH, biotinylated recombinant binding protein

Description
Alpaca anti-GFP VHH, conjugated to biotin. Compatible with avidin, streptavidin and neutravidin reagents.

Synonyms
Biotinylated GFP-Binding-Protein, biotinylated GFP-Nanobody, biotinylated anti-GFP camelid single-domain antibody fragment and biotinylated GFP-single domain antibody (sdAb)

Specificity
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and GFP-derivatives such as EGFP, CFP, YFP, BFP, and many more. For a full list, see
Fluorescent protein specificity table

Applications
ELISA, Surface Plasmon Resonance (SPR), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoelectron microscopy (Immuno EM), coating of multiwell plates

Product Size Code Price Buy
Product GFP VHH, biotinylated recombinant binding protein Size 250 µL Code gtb-250 Price $ 500
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Affinity
Dissociation constant KD of 1 pM (binding to GFP)

Specificity

GFP, EGFP, CFP, YFP, BFP and many more derivatives of GFP from Aequorea victoria

For Immunoprecipitation see: Fluorescent protein specificity table

For Immunofluorescence: eCFP, CFP, mCerulean, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine

Conjugation
Conjugated to biotin (PEG4-Biotin)

Form
Buffered solution

Protein concentration
1 g/ L

Storage buffer
Buffered aqueous solution (25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA), Preservative: 0.09% Sodium azide

Storage instructions
Shipped at ambient temperature. Store at +4°C / 40°F. Stable for one year.

Do not freeze.

Are the Nanobodies monoclonal or polyclonal?

The Nanobodies are recombinant/monoclonal.

What are the biophysical parameters of the Nanobodies?

Name MW (kDa) Molar Extinction Coefficient
(M-1 cm-1)
Theoretical pI
GFP VHH 13.9 27.055 9.4
GST VHH 14.8 28.545 8.2
Halo VHH 14.8 23.045 9.4
Histon VHH 14.4 25.565 10.2
MBP VHH 14.1 27.055 10.0
Mdm4/Hdmx VHH 13.6 14.565 10.1
MK2 VHH 14.4 32.555 9.7

mNeonGreen VHH

14.3 21.680 8.0
Myc VHH 13.5 23.045 9.6
p53 C-term VHH 14.8 34.170 9.2
p53 N-term VHH 13.0 20.065 9.7
PARP1 VHH 14.4 17.670 9.2
RFP VHH 14.9 30.035 9.3
SNAP/CLIP-tag VHH 13.1 24.075 9.8
Spot VHH (bivalent) 30.3 46.340 5.3
TurboGFP VHH 13.6 18.575 9.5
Vimentin VHH 26.7 48.150 10.0

Do the Nanobodies have any tags?

Yes, the Nanobodies have a C-terminal His6-tag.

Does the GFP VHH bind to protein A or protein G?

The GFP VHH binds to protein A, but not to protein G.

Extraordinary stable & reliable binding

  • GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate
  • Fulfills highest requirements for antibody validation
  • Structure and function are characterized

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Only for research applications, not for diagnostic or therapeutic use!