ChromoTek GFP VHH, recombinant binding protein
Alpaca anti-GFP VHH, purified recombinant binding protein
Specificity
GFP and GFP derivates
Applications
Conjugation
Type
Nanobody
Conjugate
Unconjugated
Cat no : gt
Synonyms
Validation Data Gallery
Product Information
Alpaca anti-GFP VHH, purified recombinant binding protein
Description | Alpaca anti-GFP VHH, purified recombinant binding protein for extraordinary stable & reliable binding
• GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate • Fulfills highest requirements for antibody validation • Structure and function are characterized |
Applications | Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction |
Specificity/Target | CFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine |
Conjugate | Unconjugated |
Type | Nanobody |
Class | Recombinant |
Host | Alpaca |
Affinity (KD) | Dissociation constant KD of 1 pM |
Molecular Weight | 13.9 kDa |
RRID | AB_2631361 |
Storage Buffer | 25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide |
Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year. Do not freeze. |
Documentation
SDS |
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GFP VHH, recombinant binding protein SDS (PDF) |
Datasheet |
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GFP VHH, recombinant binding protein (PDF) |
Publications
Application | Title |
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Cell Single-Molecule Nanoscopy Elucidates RNA Polymerase II Transcription at Single Genes in Live Cells. | |
Nat Methods Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging. | |
Nat Protoc Use of fluorescence-detected sedimentation velocity to study high-affinity protein interactions. | |
Dev Cell Multimerization and Retention of the Scavenger Receptor SR-B1 in the Plasma Membrane. |