Nano-Secondaries

Description
Nano-Secondaries are a novel class of secondary antibodies for higher resolution & better imaging. They consist of Nanobodies that bind their antigen with high affinity & specificity and are conjugated to Alexa Fluor® dyes.

The next level of secondary antibodies
Higher resolution in immunofluorescence
Simultaneous one-step immunostaining
Better, precise, and reproducible stainings

Applications
Immunofluorescence
Super-resolution microscopy
Western blot

Nano-Secondaries
Alpaca anti-rabbit IgG, recombinant VHH

Alpaca anti-rabbit IgG, recombinant VHH

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Code Description Size Price Qty
Code srbAF488-1-10 Description Alpaca anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 Size 10 µL Price $ 100
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Code srbAF488-1-100 Description Alpaca anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 Size 100 µL Price $ 365
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Code srbAF568-1-10 Description Alpaca anti-rabbit IgG, recombinant VHH, Alexa Fluor® 568 Size 10 µL Price $ 100
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Code srbAF568-1-100 Description Alpaca anti-rabbit IgG, recombinant VHH, Alexa Fluor® 568 Size 100 µL Price $ 365
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Code srbAF647-1-10 Description Alpaca anti-rabbit IgG, recombinant VHH, Alexa Fluor® 647 Size 10 µL Price $ 100
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Code srbAF647-1-100 Description Alpaca anti-rabbit IgG, recombinant VHH, Alexa Fluor® 647 Size 100 µL Price $ 365
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Benefits

  • Higher resolution imaging
  • Reproducible results
  • Reduced incubation and hands-on time

Higher resolution in IF: Why size matters

Nano-Secondaries are about 10 times smaller than conventional secondary IgG antibodies. Thus, these VHHs label primary antibodies with minimal fluorophore displacement for higher resolution imaging. Furthermore, our Nano-Secondaries bind the IgG in a reproduceable manner, whereas conventional polyclonal secondaries label primaries in a production lot-dependent or rather arbitrary manner. Also, the secondary Nanobodies penetrate tissues better than conventional secondary antibodies.

Fully characterized immunostaining & -blotting

Precise and reproducible staining: Recombinantly produced, stoichiometric labelled, and site-specific binding
Better imaging: High sensitivity and high affinity
Better handling: Optional one-step staining: simultaneous incubation of Nano-Secondary and primary antibody saves time

First fully validated secondaries

For the first time, ChromoTek validates secondary antibodies according to what has been suggested for primary antibodies (M. Uhlen et al. 2016):

  • Our Nano-Secondaries are tested in their target applications immunofluorescence & immunoblotting both in presence and absence of primary antibodies.
  • Cross reactivity against multiple species’ antibodies and sera has been carefully determined.
  • In addition, our secondary Nanobodies are benchmarked with established conventional secondaries.

ChromoTek’s Nano-Secondaries are based on monoclonal recombinant Nanobodies. They are always sequenced and thoroughly characterized. Their recombinant production in combination with high QC standards ensures reliable and stable Nano-Secondary products virtually without lot-to-lot variation.

 

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Only for research applications, not for diagnostic or therapeutic use!