EGFP (Enhanced Green Fluorescent Protein)
10% acrylamide gel
Purified recombinant enhanced green fluorescent protein (EGFP) fused to 6xHis-tag; Full length protein
Immunoprecipitation: use approximately 5 µg EGFP per 25 µL GFP-Trap® bead slurry
SDS-PAGE: use approximately 1 µg EGFP per lane
Western blot: use approximately 25 ng - 100 ng EGFP per lane
ELISA: coat approximately 1ng - 50 ng per well (96-well plate)
Other in vitro assays: as a fluorescent standard and for calibration
Optimal amounts/concentrations should be determined by the user.
Source: Escherichia coli
Origin: Engineered from Aequorea victoria
Liquid; buffered aqueous solution; protein concentration 1g/L
> 80% by SDS-PAGE
0.09% sodium azide
Shipped at +4°C. Store at +4°C short term (up to 1 week). Store at -20°C or -80°C long term. Avoid freeze-thaw cycles. Stable for one year.