EGFP (Enhanced Green Fluorescent Protein)

SDS-PAGE
10% acrylamide gel
Detection: InstantBlue

Description

Purified recombinant enhanced green fluorescent protein (EGFP) fused to 6xHis-tag; Full length protein

Applications

Immunoprecipitation: use approximately 5 µg EGFP per 25 µL GFP-Trap® bead slurry
SDS-PAGE: use approximately 1 µg EGFP per lane
Western blot: use approximately 25 ng - 100 ng EGFP per lane
ELISA: coat approximately 1ng - 50 ng per well (96-well plate)
Other in vitro assays: as a fluorescent standard and for calibration

Optimal amounts/concentrations should be determined by the user.

Specifications

Source: Escherichia coli
Origin: Engineered from Aequorea victoria

Form

Liquid; buffered aqueous solution; protein concentration 1g/L

Purity

> 80% by SDS-PAGE

Preservative

0.09% sodium azide

Storage instructions

Shipped at +4°C. Store at +4°C short term (up to 1 week). Store at -20°C or -80°C long term. Avoid freeze-thaw cycles. Stable for one year.

Ordering information

Prices in EUR

Product nameCodeSizePriceBuy
Enhanced Green Fluorescent Protein EGFP, purified protein EGFP-250 250µg 225 EUR Cart

Prices in USD

Product nameCodeSizePriceBuy
Enhanced Green Fluorescent Protein EGFP, purified protein EGFP-250 250µg 275 USD Cart
Protocol, SDS, etc.
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References