F2H®-Kit Basic

F2H®-Kit Basic allows effortless imaging of interactions between GFP- and RFP-tagged proteins. Study your proteins of interest and domains thereof in live mammalian cells. You just need your GFP-bait and RFP-prey plasmids ready for transfection.

F2H® principle

  • F2H® cells (BHK origin) carry a GFP-anchoring platform in the nucleus
  • GFP- and RFP-fusion proteins (bait and prey) are transiently expressed in F2H® cells
  • GFP-bait is recruited to the platform → bright green spot
  • If RFP-prey interacts with the bait → red fluorescent spot co-localizes with the green spot
  • If RFP-prey does not interact with the bait → no red spot, disperse red fluorescence

F2H® is easy

  • You get the F2H® cell line
  • You get the Platform Reagent
  • You have your GFP-bait and RFP-prey plasmids

Day 1: Transfect the bait and the prey plasmids with the Platform Reagent into the F2H® cells


Day 2: Image and quantify your results

See your proteins interact – live!

  • Visualize protein-protein interactions inside cells
  • Validate your pull-down results or Y2H hits in mammalian cells
  • Identify interacting protein domains

Please inquire if you want to learn more about our F2H® Assay Kits.

Ordering
information

Prices in EUR

ProductCodePriceBuy
F2H®-Kit Basic 
  • F2H®-BHK Cell Line (5x106 cells)
  • 100 µg Platform Reagent
  • 30 µg Control DNA
 		f2hk890 EUR Cart
Platform Reagent (100 µg)f2h-p100250 EUR Cart
Control DNA (30 µg)f2h-c3085 EUR Cart

Prices in USD

ProductCodePriceBuy
F2H®-Kit Basic 
  • F2H®-BHK Cell Line (5x106 cells)
  • 100 µg Platform Reagent
  • 30 µg Control DNA
 		f2hk1230 USD Cart
Platform Reagent (100 µg)f2h-p100345 USD Cart
Control DNA (30 µg)f2h-c30120 USD Cart
Related
information
References

Latest References

  • L. Yurlova;M. Derks;A. Buchfellner;I. Hickson;M. Janssen;D. Morrison;I. Stansfield;C. J. Brown;F. J. Ghadessy;D. P. Lane;U. Rothbauer;K. Zolghadr;E. Krausz(2014). The Fluorescent Two-Hybrid Assay to Screen for Protein-Protein Interaction Inhibitors in Live Cells: Targeting the Interaction of p53 with Mdm2 and Mdm4 J Biomol Screen. (PubMed)
  • A. Wolf;M. Mantri;A. Heim;U. Muller;E. Fichter;M. M. Mackeen;L. Schermelleh;G. Dadie;H. Leonhardt;C. Venien-Bryan;B. M. Kessler;C. J. Schofield;A. Bottger(2013). The polyserine domain of the lysyl-5 hydroxylase Jmjd6 mediates subnuclear localization Biochem J. (PubMed)
  • S. J. Wei;T. Joseph;A. Y. Sim;L. Yurlova;K. Zolghadr;D. Lane;C. Verma;F. Ghadessy(2013). In vitro selection of mutant HDM2 resistant to Nutlin inhibition PLoS One. (PubMed)
  • C. J. Brown;S. T. Quah;J. Jong;A. M. Goh;P. C. Chiam;K. H. Khoo;M. L. Choong;M. A. Lee;L. Yurlova;K. Zolghadr;T. L. Joseph;C. S. Verma;D. P. Lane(2013). Stapled Peptides with Improved Potency and Specificity that Activate p53 ACS Chem Biol. (PubMed)
  • O. Mortusewicz;E. Fouquerel;J. C. Ame;H. Leonhardt;V. Schreiber(2011). PARG is recruited to DNA damage sites through poly(ADP-ribose)- and PCNA-dependent mechanisms Nucleic Acids Res. (PubMed)


see here for complete reference list.

Only for research applications, not for diagnostic or therapeutic use.