F2H®-Kit Basic allows effortless imaging of interactions between GFP- and RFP-tagged proteins. Study your proteins of interest and domains thereof in live mammalian cells. You just need your GFP-bait and RFP-prey plasmids ready for transfection.
- F2H® cells (BHK origin) carry a GFP-anchoring platform in the nucleus
- GFP- and RFP-fusion proteins (bait and prey) are transiently expressed in F2H® cells
- GFP-bait is recruited to the platform → bright green spot
- If RFP-prey interacts with the bait → red fluorescent spot co-localizes with the green spot
- If RFP-prey does not interact with the bait → no red spot, disperse red fluorescence
F2H® is easy
- You get the F2H® cell line
- You get the Platform Reagent
- You have your GFP-bait and RFP-prey plasmids
Day 1: Transfect the bait and the prey plasmids with the Platform Reagent into the F2H® cells
Day 2: Image and quantify your results
See your proteins interact – live!
- Visualize protein-protein interactions inside cells
- Validate your pull-down results or Y2H hits in mammalian cells
- Identify interacting protein domains
Please inquire if you want to learn more about our F2H® Assay Kits.
Prices in EUR
- L. Yurlova;M. Derks;A. Buchfellner;I. Hickson;M. Janssen;D. Morrison;I. Stansfield;C. J. Brown;F. J. Ghadessy;D. P. Lane;U. Rothbauer;K. Zolghadr;E. Krausz(2014). The Fluorescent Two-Hybrid Assay to Screen for Protein-Protein Interaction Inhibitors in Live Cells: Targeting the Interaction of p53 with Mdm2 and Mdm4 J Biomol Screen. (PubMed)
- A. Wolf;M. Mantri;A. Heim;U. Muller;E. Fichter;M. M. Mackeen;L. Schermelleh;G. Dadie;H. Leonhardt;C. Venien-Bryan;B. M. Kessler;C. J. Schofield;A. Bottger(2013). The polyserine domain of the lysyl-5 hydroxylase Jmjd6 mediates subnuclear localization Biochem J. (PubMed)
- S. J. Wei;T. Joseph;A. Y. Sim;L. Yurlova;K. Zolghadr;D. Lane;C. Verma;F. Ghadessy(2013). In vitro selection of mutant HDM2 resistant to Nutlin inhibition PLoS One. (PubMed)
- C. J. Brown;S. T. Quah;J. Jong;A. M. Goh;P. C. Chiam;K. H. Khoo;M. L. Choong;M. A. Lee;L. Yurlova;K. Zolghadr;T. L. Joseph;C. S. Verma;D. P. Lane(2013). Stapled Peptides with Improved Potency and Specificity that Activate p53 ACS Chem Biol. (PubMed)
- O. Mortusewicz;E. Fouquerel;J. C. Ame;H. Leonhardt;V. Schreiber(2011). PARG is recruited to DNA damage sites through poly(ADP-ribose)- and PCNA-dependent mechanisms Nucleic Acids Res. (PubMed)
see here for complete reference list.
Only for research applications, not for diagnostic or therapeutic use.