Histone-Label* is a fluorescent chromatin probe for direct staining of chromosomes and nuclei in fixed and permeabilized cells. Histone-Label contains an antigen-binding region of a camelid single-domain antibody (VHH), specifically binding to histone H2A-H2B heterodimers. This recombinant purified VHH is chemically conjugated to the fluorescent dye ATTO488 (from ATTO-TEC) to provide a specific probe for direct immunofluorescence.
Histone-Label immunostains chromatin throughout the cell cycle (interphase chromatin, chromatin condensation and mitotic chromosomes) due to specific binding to histone H2A-H2B heterodimers. IF performance was validated on cultured human HeLa cells.
Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1/200 to 1/1000.
Form: Purified recombinant protein in PBS
Size: 10μl, 100μl
0.5 – 1mg/ml (conjugates)
Preservative: 0.09% sodium azide
Shipped at ambient temperature. Upon receipt store at 4°C. Do not freeze. Protect from light.
ChromoTek Histone-Label is a convenient, ready to use, and high-performing chromatin staining probe with low background levels and that differentiates between euchromatin and heterochromatin. HeLa cells stained in parallel with Histone-Label (1.25 mg/L) and a monoclonal rabbit anti-H2A IgG / anti-rabbit Alexa568 secondary antibody. Histone-Label co-localizes with conventional antibody staining, however Histone-Label better penetrates into the compactly packed nuclei than the anti-H2A IgG and secondary IgGs, which are one order of magnitude larger than the Histone-Label: see more green signal from Histone-Label at center of nuclei and more red signal from anti-H2A IgG on surface/edge of nuclei of merge image on very right.
- Labels Histone throughout the nuclei
- Better penetration into densly packed nuclei
Prices in EUR
*Commercialized under license of INRA, the French National Institute for Agricultural Research.