Histone-Label

Description:
Histone-Label* is a fluorescent chromatin probe for direct staining of chromosomes and nuclei in fixed and permeabilized cells. Histone-Label contains an antigen-binding region of a camelid single-domain antibody (VHH), specifically binding to histone H2A-H2B heterodimers. This recombinant purified VHH is chemically conjugated to the fluorescent dye ATTO488 (from ATTO-TEC) to provide a specific probe for direct immunofluorescence.

Specificity:
Histone-Label immunostains chromatin throughout the cell cycle (interphase chromatin, chromatin condensation and mitotic chromosomes) due to specific binding to histone H2A-H2B heterodimers. IF performance was validated on cultured human HeLa cells. 

Applications:
IF/ICC: 1/400
Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1/200 to 1/1000.


Form:
Purified recombinant protein in PBS

Size: 10μl, 100μl

Protein concentration:
0.5 – 1mg/ml (conjugates)

Preservative: 0.09% sodium azide

Storage instructions:
Shipped at ambient temperature. Upon receipt store at 4°C. Do not freeze. Protect from light.
  
 

ChromoTek Histone-Label is a convenient, ready to use, and high-performing chromatin staining probe with low background levels and that differentiates between euchromatin and heterochromatin. HeLa cells stained in parallel with Histone-Label (1.25 mg/L) and a monoclonal rabbit anti-H2A IgG / anti-rabbit Alexa568 secondary antibody. Histone-Label co-localizes with conventional antibody staining, however Histone-Label better penetrates into the compactly packed nuclei than the anti-H2A IgG and secondary IgGs, which are one order of magnitude larger than the Histone-Label: see more green signal from Histone-Label at center of nuclei and more red signal from anti-H2A IgG on surface/edge of nuclei of merge image on very right.

 

  • Labels Histone throughout the nuclei
  • Better penetration into densly packed nuclei
Ordering information

Prices in EUR

ProductCodeVolumePriceBuy
Histone-Label_Atto488tba488-1010µl75 EUR Cart
Histone-Label_Atto488tba488-100100µl270 EUR Cart
Histone-Binding protein,
purified protein for
conjugation, 1 mg/ml
tt-250250µl215 EURCart

Prices in USD

ProductCodeVolumePriceBuy
Histone-Label_Atto488tba488-1010µl100 USD Cart
Histone-Label_Atto488tba488-100100µl365 USD Cart
Histone-Binding protein,
purified protein for
conjugation, 1 mg/ml
tt-250250µl275 USDCart
Protocol, SDS, etc.
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*Commercialized under license of INRA, the French National Institute for Agricultural Research.