Vimentin-Label

Description: 
Vimentin-Label for immunostaining of vimentin intermediate filaments in fixed cells. Vimentin-Label consists of a vimentin-specific alpaca antibody domain conjugated to the fluorescent dye ATTO 488 (pdf).

Specificity:
Vimentin-Label specifically binds to the vimentin intermediate filament protein. Validated on canine MDCK cells, rodent BHK cells and on human A549 cells upon stimulation with TGFβ.

Applications:
IF/ICC: 1/200 – 1/400

Optimal working concentration is application-dependent and should be determined by testing a range of dilutions from 1/100 to 1/1000.


Size:
10 μl, 100 μl

Form: Purified recombinant protein in PBS

Preservative: 0.09% sodium azide

Protein concentration: 0.3 - 0.5 mg/ml

Storage instructions:
Shipped at ambient temperature. Upon receipt store at 4°C. Do not freeze. Protect from light.

  

Ordering information

Prices in EUR

ProductCodeVolumePriceBuy
Vimentin-Label_Atto488vba488-1010µl75 EUR Cart
Vimentin-Label_Atto488vba488-100100µl270 EUR Cart

Prices in USD

ProductCodeVolumePriceBuy
Vimentin-Label_Atto488vba488-1010µl100 USD Cart
Vimentin-Label_Atto488vba488-100100µl365 USD Cart
Protocol, SDS, etc.
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References
  • Maier J., Traenkle B., Rothbauer U. Real-time analysis of epithelial-mesenchymal transition using fluorescent single-domain antibodies. Sci Rep. 2015 Aug 21;5:13402. doi: 10.1038/srep13402. (nature)