MBP-Trap

Our MBP-Trap is a high quality MBP binding protein coupled to agarose beads for biochemical analysis of MBP fusion proteins and their interacting partners.

Fusion of proteins to E.coli Maltose binding protein (MBP) is a common technique to increase solubility and expression level of a protein. Furthermore, MBP- tagged fusion proteins are often used in protein-protein interaction studies. Both approaches require highly specific tools to isolate and detect MBP fusion proteins.

Use MBP-Trap for:

  • Immunoprecipitation (IP) / Co-IP
  • Mass spectrometry
  • Enzyme activity measurements
  • ChIP/ RIP analysis

Very high binding affinity:

With the MBP-Trap, it is possible to effectively immunoprecipitate MBP-fusion proteins at very low concentrations. See Western Blot (right): Compared to the Amylose resin, the MBP-Trap coupled to agarose beads has a considerably higher sensitivity.

Download our white paper: