mNeonGreen-Trap

ChromoTek mNeonGreen-Trap is a high quality mNeonGreen binding protein (anti-mNeonGreen VHH) coupled to a matrix (agarose beads or magnetic agarose beads). mNeonGreen-Trap is an affinity resin. It is validated, well-characterized, and optimized for biochemical analysis of mNeonGreen-fusion proteins and their interacting partners.

Use mNeonGreen-Trap for:

  • Immunoprecipitation (IP) / Co-IP
  • Mass spectrometry
  • Enzyme activity measurements
  • ChIP/ RIP analysis


mNeonGreen is a bright monomeric yellow-green fluorescent protein derived from the lancelet Branchiostoma lanceolatum. mNeonGreen fusions are used in Fluorescence, Confocal & Super Resolution Microscopy.

Available Matrices

agarose beadsmagnetic agarose beads
matrixagaroseagarose
colourwhiteblack
particle size90µm40µm
binding capacity13µg/10µl slurry12µg/10µl slurry
magneticnoyes
centrifuged up to 3000g800g

In addition, we offer non-conjugated mNeonGreen binding protein

  

mNeonGreen immunoprecipitation

Immunoprecipitation of mNeonGreen. Input (I), non-bound (FT) and bound (B) fractions were separated by SDS-PAGE followed by Coomassie staining and Western blotting. Western blot indicates high effectiveness of pull-down