Our RFP-Trap® is a high quality Red Fluorescent Protein (RFP) binding protein coupled to a monovalent matrix (agarose beads, magnetic agarose beads or magnetic particles). RFP-Trap® is optimized for biochemical analysis of RFP fusion proteins and their interacting partners.
Use RFP-Trap® for:
- Immunoprecipitation (IP) / Co-IP
- Mass spectrometry
- Enzyme activity measurements
- ChIP/ RIP analysis
The RFP-Trap® specifically binds to most common RFP derivatives:
- mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2
|agarose beads||magnetic agarose beads|
|centrifuged up to||3000g||800g|
In addition, we offer the RFP-Trap® as unconjugated protein.
Immunoprecipitations of RFP from human cells expressing RFP. Input (I), non-bound (FT) and bound (B) fractions were separated by SDS-PAGE followed by Coomassie staining and Western Blotting. The bound fraction of the conventional RFP antibodies shows in addition to RFP contaminating heavy chain and light chain. The bound fraction of the RFP-Trap® does not contain these contaminating polypeptide chains.