Spot-Tag & Spot-Nanobody
One for All: Small Affinity-Tag & Nanobody for Multiple Capture & Detection Applications
The ChromoTek Spot-System is the first peptide-tag specific Nanobody for universal capture & detection applications. It comprises Spot-Tag®, an inert 12 amino acid peptide-tag (PDRVRAVSHWSS), and the universal, rugged Spot-Nanobody that specifically binds to Spot-tagged proteins with high affinity.
Left: Interaction of Spot-Nanobody (green) with Spot Peptide
Use Spot system for
Forget about other affinity tags/antibodies: Here is Spot!
|Your Spot-Tag advantages |
compared to other antibodies:
|Your Spot-Nanobody advantages |
compared to other peptide tags:
ChromoTek's Spot related products for
- Immunoprecipitation (IP) & affinity purification use Spot-Trap®. Spot-Trap is the Spot-Nanobody coupled to agarose or magnetic agarose beads.
- Immunofluorescence (IF) & Western blotting (WB) use Spot-Label. Spot-Label is the Spot-Nanobody conjugated to fluorescent dyes. Spot-Label is the first Nanobody/peptide-tag that was applied in Super Resolution Microscopy.
- For expression of Spot-tagged fusion proteins use Spot-Tag vectors. A selection of vectors is available. Alternatively, Spot-Tag can be easily cloned by PCR primers.
- For gentle & native elution of bound Spot-tagged proteins use Spot peptide.